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Žába druhu Phyllobates terribilis, ne větší než tři centimetry, má dostatek jedu k zabití 20000 myší nebo osmi lidí. V ohrožení života totiž tyto žabky ve snaze o záchranu vylučují více svého jedu než obvykle.
V kožním sekretu pralesniček bylo nalezeno více než sto různých chemických látek, které patří do skupiny takzvaných lipofilních alkaloidů. Jsou to poměrně složité organické látky, jejichž základem je většinou šestičetná cyklická molekula obsahující pět atomů uhlíku a jeden atom dusíku. Tyto látky můžeme rozdělit do několika skupin. Ne každá pralesnička však umí vyrábět všechny typy těchto toxinů.
Přítomnost či nepřítomnost jednotlivých složek jedu slouží dnes jako klíč k rozdělení pralesniček do čtyř zoologických rodů. Takzvané histricotoxiny chybí u rodu Minyobates, zatímco vysoce účinné batrachotoxiny má jen rod Phyllobates. Rod Epipedobates postrádá histricotoxiny a kromě toho ještě skupinu toxinů zvaných 3,5-disubstituované indolyziny. Obě posledně jmenované skupiny jsou přítomné u rodu Dendrobates.
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